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Rabbit Anti Tfap2c/Ap2 Abcam Gr59885 7 At, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
Rabbit Anti Ap2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
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rabbit anti fabp4 ap2  (Cell Signaling Technology Inc)
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
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(a-d) E9.5 wild-type embryos stained for <t>AP2</t> (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).
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(a-d) E9.5 wild-type embryos stained for AP2 (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).

Journal: bioRxiv

Article Title: Lineage-specific intersection of endothelin and GDNF signaling in enteric nervous system development

doi: 10.1101/2024.02.13.580135

Figure Lengend Snippet: (a-d) E9.5 wild-type embryos stained for AP2 (a-b) and Sox10 (c-d). Magnified views of the bracketed areas including the pharyngeal arch regions in a and c are shown in b and d, respectively. (e-f) Vibratome sections of E9.75 Wnt1Cre/R26 tdTomato embryo immunostained for AP2 and Sox10. White arrows denote AP2 + ectodermal cells. Yellow arrows indicate the Wnt1Cre-labeled neural crest cell population expressing AP2 or Sox10. Magnified views of the bracketed areas in e are shown in f. (g-h) Magnified views of vibratome sections of E9.75 Pax2Cre/R26 tdTomato embryo immunostained for AP2 (g) or Sox10 (h). Yellow arrowheads point to migratory Pax2Cre-derived cells with AP2-negative nuclei. Scale bars, 200μm (e), 100μm (f-h).

Article Snippet: Antibodies used included rabbit anti-Pax2 (1:50, Invitrogen 08-1483), rabbit anti-AP2 (1:1000, Abcam ab52222), goat anti-Sox10 (1:200, Santa Cruz sc-17342), mouse anti-HuD (1:500, Santa Cruz sc-13577), mouse anti-Tuj1 (1:1000, Biolegend MMS-435P), rabbit anti-CGRP (1:500-1000, Millipore PC205L), and rabbit anti-NOS1 (1:500, Santa Cruz sc-648).

Techniques: Staining, Labeling, Expressing, Derivative Assay